Elevated Serum Reactive Oxygen Species Level Predicts Early Abortion

Joserizal Serudji, Nuzulia Irawati, Johanes Cornelius Mose, Hirowati Ali, Yusrawati Yusrawati


Background: Impaired trophoblast invasion is associated with early abortion. The calorie needed for the trophoblast cell (TC) invasion is mainly met by adenosine triphosphate (ATP) produced in the mitochondria. Reactive oxygen species (ROS), byproduct of ATP synthesis, plays an important role in cellular physiology, but a high level of ROS may result in deoxyribonucleic acid (DNA) damage or cell dysfunction, thereby impaired TC invasion leading to early abortion. The study aims to determine elevated serum ROS level to predicts early abortion.

Materials and method: This was an observational study with a cross-sectional design. Fifty subjects with gestational age less than 12 weeks, consist of 25 early abortions and 25 normal pregnancies subjects, were included in this study. Clinical examination and diagnosis are carried out in 2 Hospitals and 5 Public Health Centers in Padang. Examination of ROS levels was carried out by enzyme-linked immunosorbent assay (ELISA) in the Biomedical Laboratory, Faculty of Medicine, Universitas Andalas. The Mann-Whitney test was used to analyze the difference of serum ROS levels, with a significance level of 0.05.

Results: The subjects of the two study groups were equivalent in terms of age, gestational age, and gravidity (p=0.051, p=0.453, and p=1.000). The median ROS levels were found to be 1.36 (1.02-26.30) ng/mL in the early abortion and 1.20 (0.43-2.75) ng/mL in the normal pregnancy (p=0.003).

Conclusion: There is a significant difference between ROS levels in early abortion and normal pregnancy.

Keywords: ROS, early abortion, normal pregnancy

Full Text:



Velicky P, Knoefler M, Polheimer J. Function and control of human invasion tropjoblast subtypes: intrinsic vs maternal control. Cell Adh Migr. 2016; 10(1-2): 154-62, CrossRef.

Anun SA, Vince G, Quenby S. Trophoblast invasion. Hum Fertil. 2004; 7(3): 169-74, CrossRef.

Krumova K, Cosa G. Overview of reactive oxygen species. In: Nonell S, Flors C. Singlet Oxygen: Applications in Biosciences and Nanosciences, Volume 1. London: Royal Society of Chemistry; 2016. p.1-21, CrossRef.

Rabinovitch RC, Samborska B, Faubert B, Ma EH, Gravel S, Andrzejewski S, et al. AMPK maintains cellular metabolic homeostasis through regulation of mitochondrial reactive oxygen Species. Cell Rep. 2017; 21(1): 1-9, CrossRef.

Angelova PR, Abramov AY. Functional role of mitochondrial reactive oxygen species in physiology. Free Radic Biol Med. 2016; 100: 81-5, CrossRef.

Chamy VM, Lepe J, Catalan A, Retamal D, Escobar J, Madrid EM. Oxidative stress is closely related to severity of preeclampsia. Biol Res. 2006; 39(2): 229-36, CrossRef.

Al-Kuarishy HM, Al-Gareeb AI, Al-Maiahy. Concept and connotation of oxidative stress in preeclampsia. J Lab Physicians. 2018; 10(3): 276-82, CrossRef.

Mannaerts D, Faes E, Cos P, Briedé JJ, Gyselaers W, Cornette J, et al. Oxidative stress in healthy pregnancy and preeclampsia is linked to chronic inflammation, iron status and vascular function. PLoS One. 2018; 13(9): e0202919, CrossRef.

Shaikh SA, Vijayaraghavan R, Kumar DS, Manidip P. A comparative study of novel biomarkers on preeclampsia in relation to body mass index. Int J Res Pharm Sci. 2020; 11(1): 913-20, CrossRef.

Chakraborty D, Cui W, Rosario GX, Scott RL, Dhakal P, Renaud SJ, at al. HIF-KDM3A-MMP12 regulatory circuit ensures trophoblast plasticity and placental adaptations to hypoxia. Proc Natl Acad Sci USA. 2016; 113(46): E7212-21, CrossRef.

Wu F, Tian F and Lin Y, 2015. Oxidative stress in placenta: health and diseases. Biomed Res Int. 2015; 2015: 293271, CrossRef.

Sasaki T, Awaji T, Simada K, Sasaki H. Increased levels of reactive oxygen species in brain slices after transient hypoxia-induced by a reduced oxygen supply. Neuropsychiatry. 2018; 8(2): 684-90, CrossRef.

Turrentine JE. Clinical Protocols in Obstetrics and Gynecology. London: Informa Healthcare; 2008, CrossRef.

Golias T, Papandreou I, Denko NC. Hypoxic repression of pyruvate dehydrogenase activity is necessary for metabolic reprogramming and growth of model tumours. Sci Rep. 2016; 6: 31146, CrossRef.

Anin SA, Vince G, Quenby S. Trophoblast invasion. Hum Fertil. 2004; 7(3): 169-74, CrossRef.

Horii M, Li Y, Wakeland AK, Pizzo DP, Nelson KK, Sabatini K, et al. Human pluripotent stem cells as a model of trophoblast differentiation in both normal development and disease. Proc Natl Acad Sci USA. 2016; 113(27): E3882-91, CrossRef.

Thomas LW, Ashcroft M. Exploring the molecular interface between hypoxia-inducible factor signalling and mitochondria. Cell Mol Life Sci. 2019; 76(9): 1759-77, CrossRef.

Chan SY, Zhang YY, Loscalzo J. MicroRNA-210 controls mitochondrial metabolism during hypoxia by repressing the iron-sulfur cluster assembly proteins ISCU1/2. Cell Metab. 2009; 10(4): 273-84, CrossRef.

Guan Y, Song X, Sun W, Wang Y, Liu B. Effect of hypoxia-induced microRNA-210 expression on cardiovascular disease and the underlying mechanism. Oxid Med Cell Longev. 2019; 2019: 4727283, CrossRef.

Bilici M. The importance of oxidative stress in early week pregnancy loss. Crescent J Med Biol Sci. 2014; 1(4): 151-3, article.

DOI: https://doi.org/10.21705/mcbs.v5i1.192

Indexed by:




Cell and BioPharmaceutical Institute