Investigation on Cell Surface Markers of Dental Pulp Stem Cell Isolated from Impacted Third Molar Based on International Society for Cellular Therapy Proposed Mesenchymal Stem Cell Markers

Ferry Sandra, Janti Sudiono, Yohanna Feter, Nadhia Sari Afiana, Jessica Nathalia Chandra, Kharima Abdullah, Jasmine Shafira, Angliana Chouw


Background: Recently we have isolated and cultured dental pulp stem cell (DPSC) derived from impacted third molar (DPSC-M3). The DPSC-M3 was suggested as mesenchymal stem cell, however the cell surface markers were not completely clarified. Therefore current study was conducted to investigate the markers.

Materials and Methods: Passage 5 DPSC-M3 was cultured, labeled and examined with flow cytometer. All markers were investigated according to the proposed cell surface marker panel for the minimal identification of human mesenchymal stem cell (MSC) by International Society for Cellular Therapy (ISCT). The positive markers were cluster of differentiation (CD)90, CD73, CD105, while the negative markers were CD34, CD45, CD11b, CD19, and Human Leukocyte Antigen (HLA)-DR. 

Results: Results showed that the size and granularity of DPSC-M3 were ranged from 75 to 230 and 27 to 203, respectively. The cell surface antigens examination showed that CD90, CD105 and CD73 were highly expressed (>95%), meanwhile expressions of CD45, CD34, CD11b, CD19 and HLA-DR were <2%.

Conclusion: Since the all markers expression were in accordance to the proposed cell surface marker panel for the minimal identification of human MSC by ISCT, DPSC-M3 could be suggested as an MSC.

Keywords: dental pulp, stem cell, dental pulp stem cell, ISCT, flow cytometry

Full Text:



Sjamsuhidajat, Sandra F, Tarwadi, Sardjono CT, Widyawati H, Ismail, et al. Pedoman Riset Sel Punca Manusia. Jakarta: Asosiasi Sel Punca Indonesia; 2010, Link.

Sandra F, Sidiq KM, Halim D. Terapi Stem Cell – Terobosan Kedokteran Modern untuk Penderita Gagal Jantung. Jakarta: Grasindo; 2012, Link.

Halim D, Murti H, Sandra F, Boediono A, Djuwantono T, Setiawan B. Stem Cell Dasar Teori & Aplikasi Klinis. Jakarta: Penerbit Erlangga; 2010, Link.

Chalisserry EP, Nam SY, Park SH, Anil S. Therapeutic Potential of Dental Stem Cells. J Tissue Eng. 2017; 8: 2041731417702531, CrossRef.

Mao X, Liu Y, Chen C. Mesenchymal Stem Cells and Their Role in Dental Medicine. Dent Clin NA. 2017; 61(1): 161-72, CrossRef.

Potdar PD, Jethmalani YD. Human Dental Pulp Stem Cells: Applications in Future Regenerative Medicine. World J Stem Cells. 2015; 7(5): 839-51, CrossRef.

Aini N, Setiawan B, Sandra F. Karakteristik Biologis dan Diferensiasi Stem Cell: Fokus pada Mesenchymal Stem Cell. CDK. 2008; 35(161): 64-7, Link.

Ryan JM, Barry FP, Murphy JM, Mahon BP. Mesenchymal Stem Cells Avoid Allogeneic Rejection. J Inflamm. 2005; 2(1): 8, CrossRef.

Widowati W, Sardjono CT, Wijaya L, Laksmitawati DR, Sandra F. Extract of Curcuma longa L. and (-)-Epigallo Catechin-3-Gallate Enhanced Proliferation of Adipose Tissue–derived Mesenchymal Stem Cells (AD-MSCs) and Differentiation of AD-MSCs into Endothelial Progenitor Cells. J USA-China Med Sci. 2012; 9(1): 22-9, CrossRef.

Sandra F, Sudiono J, Sidharta EA, Sunata EP, Sungkono DJ, Dirgantara Y, Chouw A. Conditioned Media of Human Umbilical Cord Blood Mesenchymal Stem Cell-derived Secretome Induced Apoptosis and Inhibited Growth of HeLa Cells. Indones Biomed J. 2014; 6(1): 57-62, CrossRef.

Lubis AM, Sandhow L, Lubis VK, Noor A, Gumay F, Merlina M, et al. Isolation and Cultivation of Mesenchymal Stem Cells from Iliac Crest Bone Marrow for Further Cartilage Defect Management. Acta Med Indones. 2011; 43(3): 178-84, Link.

Hass R, Kasper C, Böhm S, Jacobs R. Different Populations and Sources of Human Mesenchymal Stem Cells (MSC): A Comparison of Adult and Neonatal Tissue-derived MSC. Cell Commun Signal. 2011; 9(1): 12, CrossRef.

Sudiono J, Oka CT, Djamil MS, Sandra F. Regenerative Medicine in Dental and Oral Tissues: Dental Pulp Mesenchymal Stem Cell. Padjadjaran J Dent. 2016; 28(1): 31-7, CrossRef.

Yu T, Volponi AA, Babb R, An Z, Sharpe PT. Stem Cells in Tooth Development, Growth, Repair, and Regeneration. Curr Top Dev Biol. 2015; 115: 187-212, CrossRef.

Ledesma-martínez E, Mendoza-núñez VM. Mesenchymal Stem Cells Derived from Dental Pulp: A Review. Stem Cells Int. 2016; 2016: 4709572, CrossRef.

Alge DL, Zhou D, Adams LL, Wyss BK, Shadday MD, Woods EJ, et al. Donor-matched Comparison of Dental Pulp Stem Cells and Bone Marrowderived Mesenchymal Stem Cells in A Rat Model. J Tissue Eng Regen Med. 2010; 4(1): 73-81, CrossRef.

Atalayin C, Tezel H, Dagci T, Karabay Yavasoglu NU, Oktem G, Kose T. In vivo Performance of Different Scaffolds for Dental Pulp Stem Cells Induced for Odontogenic Differentiation. Braz Oral Res. 2016; 30(1): e120, CrossRef.

Bakopoulou A, About I. Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application. Stem Cells Int. 2016; 2016: 4209891, CrossRef.

Feter Y, Afiana NS, Chandra JN, Abdullah K, Shafira J, Sandra F. Dental Mesenchymal Stem Cell: Its Role in Tooth Development, Types, Surface Antigens and Differentiation Potential. Mol Cell Biomed Sci. 2017; 1(2): 50-7, CrossRef.

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal Criteria for Defining Multipotent Mesenchymal Stromal Cells. The International Society for Cellular Therapy Position Statement. Cytotherapy. 2006; 8(4): 315-7, CrossRef.

Sandra F, Sudiono J, Tri C, Binartha O, Chouw A, Djamil MS. Growth and Osteogenic Differentiation of CD117+ Dental Pulp and Periodontal Ligament Cells. Indones Biomed J. 2017; 9(2): 78-83, CrossRef.

Majore I, Moretti P, Hass R, Kasper C. Identification of Subpopulations in Mesenchymal Stem Cell-like Cultures from Human Umbilical Cord. Cell Commun Signal. 2009; 7: 6, CrossRef.

Madeira A, Silva CLD, Santos FD, Camafeita E, Cabral JMS, Correia IS. Human Mesenchymal Stem Cell Expression Program upon Extended ex-vivo Cultivation, as Revealed by 2-DE-based Quantitative Proteomics. PLOS ONE. 2012; 7(8): e43523, CrossRef.

Yalvac ME, Ramazanoglu M, Rizvanov AA, Sahin F, Bayrak OF, Salli U, et al. Isolation and Characterization of Stem Cells Derived from Human Third Molar Tooth Germs of Young Adults: Implications in Neo-vascularization, Osteo-, Adipo- and Neurogenesis. Pharmacogenomics J. 2010; 10(2): 105-13, CrossRef.

Sandra F, Lahirin R. Human Umbilical Cord Blood Serum Has Higher Potential in Inducing Proliferation of Fibroblast than Fetal Bovine Serum. Mol Cell Biomed Sci. 2017; 1(2): 65-9, CrossRef.

Sandra F, Murti H, Aini N, Sardjono C, Setiawan B. Potensi Terapi Sel Punca dalam Dunia Kedokteran dan Permasalahannya. JKM. 2008; 8(1): 94-101, Link.

Oktaviono YH, Sargowo D, Widodo MA, Dirgantara Y, Chouw A, Sandra F. Proliferation of Peripheral Blood-derived Endothelial Progenitor Cells from Stable Angina Subjects. Indones Biomed J. 2014; 6(2): 91-6, CrossRef.

Sandra F, Oktaviono YH, Widodo MA, Dirgantara Y, Chouw A, Sargowo D. Endothelial Progenitor Cells Proliferated via MEK-dependent p42 MAPK Signaling Pathway. Mol Cell Biochem. 2015; 400(1-2): 201-6, CrossRef.

Oktaviono YH, Sargowo D, Widodo MA, Dirgantara Y, Chouw A, Sandra F. Role of Signal Transduction Erk1/2 on The Proliferation of Endothelial Progenitor Cell (EPC) of Patients with Stable Angina Pectoris Induced by Growth Factors. Indones J Clin Pathol Med Lab. 2016; 22(3): 219-26, CrossRef.

Widowati W, Mozef T, Risdian C, Ratnawati H, Tjahjani S, Sandra F. The Comparison of Antioxidative and Proliferation Inhibitor Properties of Piper betle L., Catharanthus roseus [L] G.Don, Dendrophtoe petandra L., Curcuma mangga Val. Extracts on T47D Cancer Cell Line. Int Res J Biochem Bioinform. 2011; 1(2): 22-8, Link.

Sandra F, Munir D. Herbal medicine induces circulation and proliferation of Endothelial Progenitor Cell (EPC). Stem Cell Oncology. 2018; 2018: 103-7, CrossRef.

Sandra F. Role of Herbal Extract in Stem Cell Development. Mol Cell Biomed Sci. 2018; 2(1): 19-22, CrossRef.


Copyright (c) 2019 Cell and BioPharmaceutical Institute

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Indexed by:



Cell and BioPharmaceutical Institute