Soymilk Formula Increases Estrogen and Reduces Testosterone Level in Male Infant White Wistar Rats

Eveline Margo, Wimpie Pangkahila, I Gusti Made Aman

Abstract


Background: Children’s immunity system is relatively lower on first year of life, causing atopic babies, leading to allergy. Ideally, newborn babies are given breast milk as main food source on the first 6 months, but often being replaced with cow milk which can induce Cow’s Milk Protein Allergy. For the alternative can replace it with soymilk formula which contains phytoestrogen from isoflavon, which works just like estrogen. The aim of this study to determine whether administration of soymilk formula is capable on increasing estrogen level and reducing testosterone level on male infant white Wistar rats (Rattus norvegicus).

Materials and Methods: This research used post test only control group design. Samples were consisted of 36 infant male white Wistar rats aged 7 days, weighing from 10-15 grams, divided into 2 groups, control group which was given cow’s milk and intervention group which was given soymilk. Each group were given intervention with the dose 5% of BW (grams), given twice-a-day orally for 21 days, and subsequently in the morning their blood samples were taken to examine the level of estrogen and testosterone hormone.

Results: The study showed that the level of estrogen on intervention group was significantly higher than the control group with mean estrogen level of 0.55±0.03 pg/mL in the control group and 0.82±0.01 pg/mL in the intervention group, while the mean testosterone level was significantly lower on intervention group compared with control group (p<0.05) with 3.25±0.15 ng/mL on control group and 2.36±0.22 ng/mL on intervention group.

Conclusion: Soymilk was able to increase estrogen level and reduce testosterone level on male infant white Wistar rats (Rattus norvegicus).

Keywords: soymilk formula, estrogen, testosterone, male infant rats


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References


Kim SH, Park MJ. Effects of phytoestrogen on sexual development. Korean J Pediatr. 2012; 55(8): 265-71, CrossRef.

Munasir Z, Siregar SP. Alergi susu sapi. In: Alergi-Imunologi Anak. 2nd ed. Jakarta: Badan Penerbit IDAI; 2010. p.284-93.

Koletzko S, Niggemann B, Arato A, Dias JA, Heuschkel R, Husby S, et al. Diagnostic approach and management of cow’s milk protein allergy in infants and children: ESPGHAN GI Committee practical guidelines. J Pediatr Gastroenterol Nutr. 2012; 55(2): 221-9, CrossRef.

Chen A, Rogan WJ. Isoflavones in soy infant formula: a review of evidence for endocrine and other activity in infants. Annu Rev Nutr. 2004; 24: 33-54, CrossRef.

Cao Y, Calafat AM, Doerge DR, Umbach DM, Bernbaum JC, Twaddle NC, et al. Isoflavone in urine, saliva, and blood of infants: data from a pilot study on the estrogenic activity of soy formula. J Expo Sci Environ Epidemiol. 2009; 19(2): 223-34, CrossRef.

Dinsdale EC, Ward WE. Early Exposure to soy isoflavones and effects on reproductive health: a review of human and animal studies. Nutrients. 2010; 2(11): 1156-87, CrossRef.

Leung A, Otley A. Concerns for the use of soy-based formulas in infant nutrition. Peadiatr Child Health. 2009; 14(3): 109-13, CrossRef.

Strom BL, Schinnar R, Zieger EE, Barnhart KT, Sammel MD, et al. Exposure to soy-based formula in infancy and endocrinological and reproductive outcomes in young adulthood. JAMA. 2001; 286(7): 807-14, CrossRef.

Hughes I. Variability and Uncertainty inToxicology of Chemicals in Food, Consumer Products and the Environment. London: Committee on Toxicity of Chemicals in Food, Consumer Products and the Environment; 2007, Link.

Linus Pauling Institute, Oregon State University [Internet]. Drake VJ. Isoflavones [updated 2009 Dec; cited 2015 Aug 30]. Available from: https://lpi.oregonstate.edu/.

Braunstein GD. Testes. In: Gardner DG, Shoback D, editors. Greenspan’s: Basic & Clinical Endocrinology. 9th ed. New York: McGraw-Hill; 2011. p.395-401, Link.

Robertson KM, O’Donnell L, Simpson ER, Jones ME. The phenotype of aromatase knockout mouse reveals dietary phytoestrogens impact significantly on testis function. Endrocrinology. 2002; 143(8): 2913-21, CrossRef.

American Fancy Rat & Mouse Association [Internet]. Robbins K. Caring for Rat & Mouse Orphans [updated 1998; cited 2015 Oct 27]. Available from: http://www.afrma.org/.

Widiartini W, Siswati E, Setiyawati A, Rohmah IM, Prastyo E. Pengembangan Usaha Produksi Tikus Putih (Rattus norvegicus) Tersertifikasi Dalam Upaya Memenuhi Kebutuhan Hewan Laboratorium. e-Proceedings PIMNAS. 2013: PKM-K; 1-8, Link.

Kumar R. Testis. In: Tunru ISA, editor. Dasar-dasar Patofisiologi Penyakit. Tangerang: Binarupa Aksara Publisher; 2013. p.462-8.




DOI: https://doi.org/10.21705/mcbs.v3i1.40

Copyright (c) 2019 Eveline Margo

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Cell and BioPharmaceutical Institute