Caffeic Acid Induced Apoptosis in MG63 Osteosarcoma Cells Through Activation of Caspases
Abstract
Background: Caffeic acid has been reported that when it is combined with all-trans retinoic acid, it can inhibit proliferation activity of SaOS-2 or OSA-01 cells. In addition, caffeic acid merely could reduce cell viability of SaOS-2 cells. However, there is not any study in caffeic acid's possible effect to induce apoptosis in osteosarcoma cell.
Materials and Methods: MG-63 cells were cultured in Dulbecco’s Modified Eagle Medium containing 10% fetal bovine serum. Cells were treated with various concentrations of caffeic acid. Apoptosis were analyzed with Sub-G1 assay and activation of caspase-8, -9, and -3 were analyzed with immunoblotting. Caffeic acid-induced percentage of apoptotic cells and cleaved-8, -9, -3 were then statistically analyzed.
Results: Sub-G1 results showed that caffeic acid significantly induced apoptosis in MG-63 osteosarcoma cells in concentration dependent manner. Immunoblotting results showed that caffeic acid induced cleavage of caspase-8, -9 and -3. Cleaved-caspase-8 and -9 were increased at 1-hour treatment of caffeic acid, while cleaved-caspase 3 was increased markedly at 6-hours treatment of caffeic acid.
Conclusions: Caffeic acid induces apoptosis significantly in concentration dependent manner through caspase-dependent intrinsic apoptotic pathway.
Keywords: caffeic acid, osteosarcoma, MG-63, apoptosis, caspase
Full Text:
PDFReferences
Shaikh AB, Li F, Li M, He B, He X, Chen G, et al. Present Advances and Future Perspectives of Molecular Targeted Therapy for Osteosarcoma. Int J Mol Sci. 2016; 17(4): 506. doi: 10.3390/ijms17040506. CrossRef
Anderson ME. Update on Survival in Osteosarcoma. Orthop Clin North Am. 2016; 47(1): 283-92. CrossRef
Longhi A, Ferrari S, Bacci G, Specchia S. Long-Term Follow-Up of Patients with Doxorubicin-Induced Cardiac Toxicity after Chemotherapy for Osteosarcoma. Anticancer Drugs. 2007; 18(6): 737-44. CrossRef
Zhou W, Hao M, Du X, Chen K, Wang G, Yang J. Advances in Targeted Therapy for Osteosarcoma. Discov Med. 2014; 17(96): 301-7. Link
Wu CC, Cheng CH, Lee YH, Chang IL, Chen HY, Hsieh CP, et al. Ursolic Acid Triggers Apoptosis in Human Osteosarcoma Cells via Caspase Activation and the ERK1/2 MAPK Pathway. J Agric Food Chem. 2016; 64(21): 4220-6. CrossRef
Wang T, Gong X, Jiang R, Li H, Du W, Kuang G. Ferulic Acid Inhibits Proliferation and Promotes Apoptosis via Blockage of PI3K/Akt Pathway in Osteosarcoma Cell. Am J Transl Res. 2016; 8(2): 968-80. Link
Ge Y, Wang Y, Pang L, Zhang L, Zhai Y, Zhou H. Proliferation, Apoptosis and Invasion Effects of Mistletoe Alkali on Human Osteosarcoma U2OS In Vitro. Int Surg. 2016 Apr 25. [Epub ahead of print] CrossRef
Sandra F. Kukita T, Tang QY, Iijima T. Cafeic Acid Inhibits NFkappaB Activation of Osteoclastogenesis Signaling Pathway. Indones Biomed J. 2011; 3(3): 216-22. CrossRef
Tang QY, Kukita T, Ushijima Y, Kukita A, Nagata K, Sandra F, et al. Regulation of Osteoclastogenesis by Simon Extracts Composed of Caffeic Acid and Related Compounds: Successful Suppression of Bone Destruction Accompanied with Adjuvant-Induced Arthritis in Rats. Histochem Cell Biol. 2006; 125(3): 215-25. CrossRef
Sandra F, Kukita T, Muta T, Iijima T. Caffeic Acid Inhibited Receptor Activator of Nuclear Factor κB Ligand (RANKL)-Tumor Necrosis Factor (TNF) α-TNF Receptor Associated Factor (TRAF) 6 Induced Osteoclastogenesis Pathway. Indones Biomed J. 2013; 5(3): 173-8. CrossRef
Chang HT, Chen IL, Chou CT, Liang WZ, Kuo DH, Shieh P, et al. Effect of Caffeic Acid on Ca(2+) Homeostasis and Apoptosis in SCM1 Human Gastric Cancer Cells. Arch Toxicol. 2013; 87(12): 2141-50. CrossRef
Chang WC, Hsieh CH, Hsiao MW, Lin WC, Hung YC, Ye JC. Caffeic Acid Induces Apoptosis in Human Cervical Cancer Cells Through the Mitochondrial Pathway. Taiwan J Obstet Gynecol. 2010; 49: 419-24. CrossRef
Krzyzankova M, Chovanova S, Chlapek P, Radsetoulal M, Neradil J, Zitterbart K, et al. LOX/COX Inhibitors Enhance the Antineoplastic Effects of All-Trans Retinoic Acid in Osteosarcoma Cell Lines. Tumour Biol. 2014; 35(8): 7617-27. CrossRef
Sandra F, Khosravi-Far R. Survivin S81A Enhanced TRAIL's Activity in Inducing Apoptosis. Indones Biomed J. 2010; 2(3): 113-7. CrossRef
Sandra F, Sudiono J, Sidharta EA, Sunata EP, Sungkono DJ, Dirgantara Y, et al. Conditioned Media of Human Umbilical Cord Blood Mesenchymal Stem Cell-Derived Secretome Induced Apoptosis and Inhibited Growth of HeLa Cells. Indones Biomed J. 2014; 6(1): 57-62. CrossRef
Sandra F, Hendarmin L, Kukita T, Nakao Y, Nakamura N, Nakamura S. Ameloblastoma Induces Osteoclastogenesis: a Possible Role of Ameloblastoma in Expanding in the Bone. Oral Oncol. 2005; 41(6): 637-44. CrossRef
Tang CH, Chuang JY, Fong YC, Maa MC, Way TD, Hung CH. Bone-Derived SDF-1 Stimulates IL-6 Release via CXCR4, ERK and NF-kappaB Pathways and Promotes Osteoclastogenesis in Human Oral cancer cells. Carcinogenesis. 2008; 29(8): 1483-92. CrossRef
Costa-Rodrigues J, Teixeira CA, Fernandes MH. Paracrine-Mediated Osteoclastogenesis by the Osteosarcoma MG63 Cell Line: is RANKL/RANK Signalling Really Important?. Clin Exp Metastasis. 2011; 28(6): 505-14. CrossRef
Taylor R, Knowles HJ, Athanasou NA. Ewing Sarcoma Cells Express RANKL and Support Osteoclastogenesis. J Pathol. 2011; 225(2): 195-202. CrossRef
Fischer U, Jänicke RU, Schulze-Osthoff K. Many Cuts to Ruin: a Comprehensive Update of Caspase Substrates. Cell Death Differ. 2003; 10(1): 76-100. CrossRef
Reyland ME. Protein Kinase Cdelta and Apoptosis. Biochem Soc Trans. 2007; 35(5): 1001-4. CrossRef
Sandra F, Hendarmin L, Nakao Y, Nakamura N, Nakamura S. TRAIL Cleaves Caspase-8, -9 and -3 of AM-1 Cells: a Possible Pathway for TRAIL to Induce Apoptosis in Ameloblastoma. Tumour Biol. 2005; 26(5): 258-64. CrossRef
Sandra F, Degli Esposti M, Ndebele K, Gona P, Knight D, Rosenquist M, et al. Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Alters Mitochondrial Membrane Lipids. Cancer Res. 2005; 65(18): 8286-97. CrossRef
DOI: https://doi.org/10.21705/mcbs.v1i1.6
Copyright (c) 2017 Cell and BioPharmaceutical Institute
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Indexed by:
Cell and BioPharmaceutical Institute
