The Effect of Immobilization Stress on Gastric Mucosal Histopathology in White Mice (Mus musculus) Male Swiss Webster Strain

Rizki Bunaya, Ilhami Romus, Fajri Marindra Siregar, Desby Juananda


Background: Immobilization stress is one method of stress induction on experimental animals. It affects the psychology and physical of experimental animals and is the recommended method for assessing changes in histological structure damage. The purpose of research was to analyze the effect of immobilization stress on gastric mucosal in mice.

Materials and Methods: This research was experimental with post-test-only control group design. Twenty white mice (Mus musculus) male Swiss Webster strains were used in this study and divided into 4 groups: control, immobilization stress 14 days, immobilization stress 21 days, immobilization stress 28 days. Mice were given immobilization stress using 50 cc syringes for 2 hours every day for 14 days, 21 days and 28 days. Gastric mucosal damage in mice was analyzed under a microscope with of 10 fields of view in each sample. Data were analyzed using the Kruskal Wallis test and Mann Whitney test.

Results: Gastric mucosal damage score were 0 in control, 1.42±0.265 in 14 days, 1.82±0.265 in 21 days, and 2.54±0.05 in 28 days. There was significant difference between each group (p<0.05), while the greatest damage was found in the 28 days group.

Conclusion: These result indicated that immobilization stress caused gastric mucosal damage and the degree of damage is in accordance with duration of stress.

Keywords: gastric mucosal, immobilization, stress

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Garcia C, Melo-thomas L, Spadari-bratfisch RC, Ortolani D. Stress-induced endocrine response and anxiety: the effects of comfort food in rats. Stress. 2014; 17(3): 211-8, CrossRef.

Kranner I, Minibayeva F V, Beckett RP, Seal CE. What is stress? Concepts, definitions and applications in seed science. New Phytol. 2010; 188(3): 655-73, CrossRef.

Marten F. The Mediating Effect of Eustress and Distress on the Relation between the Mindset Towards Stress Health [Essay]. Enschede: University of Twente; 2017, article.

Koolhaas JM, Bartolomucci A, Buwalda B, Boer SF De, Flügge G, Korte SM, et al. Neuroscience and biobehavioral reviews stress revisited: A critical evaluation of the stress concept. Neurosci Biobehav Rev. 2011; 35(5): 1291-301, CrossRef.

Armario A. Studying Chronic Stress in Animals: Purposes, Models and Consequences. New Jersey: John Wiley & Sons Ltd; 2015.

Konturek PC, Brzozowsk T, Konturek SJ. Stress and the gut: pathophysiology, clinical consequences, diagnostic approaach and treatment options J Physiol Pharmacol. 2011; 62(6): 591-9, article.

Foster JA, Rinaman L, Cryan JF. Stress & the gut-brain axis: Regulation by the microbiome. Neurobiol Stress. 2017; 7: 124-36, CrossRef.

Plummer MP, Blaser AR, Deane AM. Stress ulceration: prevalence, pathology and association with adverse outcomes. BioMed Central. 2014; 18(2): 213, CrossRef.

Reber SO, Slattery DA. Editorial: Using stress-based animal models to understand the mechanisms underlying psychiatric and somatic disorders. Front Psychiatry. 2016; 7: 192, CrossRef.

Brainova I. Effects of immobilization stress on distribution of nadph-d reactive neurons in rat’s paraventricular nucleus. IJTRA. 2015; 3(6): 150-3, article.

Altayeb ZM, Salem MM. Light and electron microscopic study on the effect of immobilization stress on adrenal cortex of adult rats and possible ameliorative role of vitamin E. Neuroscience. 2017; 1(1): 44-56, CrossRef.

Koolhaas JM, Boer SF, Buwalda B. Stress and adaptation. Toward ecologically relevant animal model. Physiol Sci. 2006; 15(3): 109-12, CrossRef.

Wang Y, Liu J, Yang ZY. Effects of intestinal mucosal blood flow and motility on intestinal mucosa. World J Gastroenterol. 2011; 17(5): 657-61, CrossRef.

Kawano S, Tsuji S. Role of mucosal blood flow: a conceptional review in gastric mucosal injury and protection. J Gastroenterol Hepatol. 2000; 15(S1): D1-6, CrossRef.


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Cell and BioPharmaceutical Institute