Curcuma mangga Val. Extract as Antidiabetic Agent in 3T3-L1 Adipocyte Cells

Dwiyati Pujimulyani, Wisnu Adi Yulianto, Astuti Setyawati, Rizal Rizal, Rismawati Laila Qodariah, Zakiyatul Khoiriyah, Annisa Arlisyah, Wahyu Widowati

Abstract


Background: With the increase of diabetes mellitus (DM) prevalence, natural product emerged as complementary source on the development of new drug for this disease. White saffron (Curcuma mangga Val.) is a widely available plant found in Indonesia which often used traditionally as medicine for various ailment. Unfortunately scientific evidence of its antidiabetic activity has not been described very well. Present study was trying to evaluate the antidiabetic potential of white saffron based on the change of lipid accumulation.

Materials and Methods: Cells viability assay was done using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) reagent to determine the safe concentrations of C. mangga Val. extract and its fractions including hexane, ethyl acetate, butanol, ethanol, water fractions and curcumol for the further assay. The preadipocyte cells (3T3-L1) were grown and differentiated into adipocyte cells using 3-isobutyl-1-methylxanthine (IBMX), dexamethasone and insulin. The adipocyte cells were treated with C. mangga Val. extract (CME) (the safest fraction at all concentrations) for 24 h. Oil red O staining was used to measure the lipid accumulation in adipocyte cells.

Results: The CME was not toxic and able to decrease the lipid droplets of the 3T3-L1 adipocyte cells.

Conclusion: The CME has potential antidiabetic activity due to ability to decrease the lipid droplet without disturbing the viability of the 3T3-L1 adipocyte cells.

Keywords: white saffron, Curcuma mangga Val., antidiabetic


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References


Spoor DCA, Martineau LC, Leduc C, Benhaddou-Andaloussi A, Meddah B, Harris C, et al. Selected plant species from the Cree pharmacopoeia of northern Quebec possess antidiabetic potential. Can J Physiol Pharmacol. 2006; 84(8-9): 847-58, CrossRef.

Agrawal R, Sethiya NK, Mishra SH. Antidiabetic activity of alkaloids of Aerva lanata roots on streptozotocin-nicotinamide induced type-II diabetes in rats. Pharm Biol. 2013; 51(5): 635-42, CrossRef.

Srirod S, Tewtrakul S. Anti-inflammatory and wound healing effects of cream containing Curcuma mangga extract. J Ethnopharmacol. 2019; 238: 111828, CrossRef.

ASEAN Secretariat. ASEAN Herbal and Medicinal Plants. Jakarta: ASEAN Secretariat; 2010.

Pujimulyani D, Raharjo S, Marsono Y, Santoso U. The effects of blanching treatment on the radical scavenging activity of white saffron (Curcuma mangga Val.). Int Food Res J. 2010; 17: 615-21, article.

Pujimulyani D, Raharjo S, Marsono Y, Santoso U. The phenolic substances and antioxidant activity of white saffron (Curcuma mangga Val.) as affected by blanching methods. Int J Nutr Food Eng. 2013; 7(10): 947-50, article.

Afzal A, Oriqat G, Akram Khan M, Jose J, Afzal M. Chemistry and biochemistry of terpenoids from curcuma and related species. J Biol Act Prod from Nat. 2013; 3(1): 1-55, CrossRef.

Goel A, Kunnumakkara AB, Aggarwal BB. Curcumin as “curecumin”: from kitchen to clinic. Biochem Pharmacol. 2008; 75(4): 787-809. Goel A, Kunnumakkara AB, Aggarwal BB. Curcumin as “curecumin”: from kitchen to clinic. Biochem Pharmacol. 2008; 75(4): 787-809, CrossRef.

Shehzad A, Ha T, Subhan F, Lee YS. New mechanisms and the anti-inflammatory role of curcumin in obesity and obesity-related metabolic diseases. Eur J Nutr. 2011; 50(3): 151-61, CrossRef.

Pujimulyani D, Yulianto WA, Setyowati A, Arumwardana S, Rizal R. Antidiabetic and antioxidant potential of Curcuma mangga Val. extract and fractions. Asian J Agric Biol. 2018; 6(2): 162-8, article.

Wong CP, Kaneda T, Morita H. Plant natural products as an anti-lipid droplets accumulation agent. J Nat Med. 2014; 68(2): 253-66, CrossRef.

Wakabayashi I, Daimon T. A strong association between lipid accumulation product and diabetes mellitus in japanese women and men. J Atheroscler Thromb. 2014; 21(3): 282-8, CrossRef.

Mirmiran P, Bahadoran Z, Azizi F. Lipid accumulation product is associated with insulin resistance, lipid peroxidation, and systemic inflammation in type 2 diabetic patients. Endocrinol Metab. 2014; 29(4): 443, CrossRef.

Widowati W, Rani AP, Hamzah RA, Arumwardana S, Afifah E, Kusuma HSW, et al. Antioxidant and antiaging assays of Hibiscus sabdariffa extract and its compounds. Nat Prod Sci. 2017; 23(3): 192, CrossRef.

Widowati W, Widyanto RM, Husin W, Ratnawati H, Laksmitawati DR, Setiawan B, et al. Green tea extract protects endothelial progenitor cells from oxidative insult through reduction of intracellular reactive oxygen species activity. Iran J Basic Med Sci. 2014; 17(9): 702-9, article.

Sandhiutami NMD, Moordiani M, Laksmitawati DR, Fauziah N, Maesaroh M, Widowati W. In vitro assesment of anti-inflammatory activities of coumarin and Indonesian cassia extract in RAW264.7 murine macrophage cell line. Iran J Basic Med Sci. 2017; 20(1): 99-106, article.

Widowati W, Darsono L, Suherman J, Afifah A, Rizal R, Arinta Y, et al. Mangosteen peel extract (Garcinia mangostana L.) and its constituents to lower lipid content on adipogenesis cells model (3T3-L1). J Nat Remedies. 2018; 18(2): 41-8, CrossRef.

Hidayat M, Soeng S, Prahastuti S, Erawijantari P, Widowati W. Inhibitory potential of ethanol extract of detam 1 soybean (Glycine max) seed and jati belanda (Guazuma ulmifolia) leaves on adipogenesis and obesity models in 3T3-L1 cell line. J Sci Res Rep. 2015; 6(4): 304-12, CrossRef.

Hidayat M, Prahastuti S, Fauziah N, Maesaroh M, Balqis B, Widowati W. Modulation of adipogenesis-related gene expression by ethanol extracts of Detam 1 soybean and Jati belanda leaf in 3T3-L1 cells. Bangladesh J Pharmacol. 2016; 11(3): 697, CrossRef.

Mutai E, Sunkara R, Vizcarra J, Walker LT, Verghese M. Antioxidant, enzyme inhibitory and anti-obesity potential of sorrel calyx extracts in 3T3-L1 adipocytes. Food Nutr Sci. 2015; 6(5): 452-65, CrossRef.

Tiwari AK, Madhusudanarao J. Diabetes mellitus and multiple therapeutic approaches of phytochemicals: Present status and future prospects. Curr Sci. 2002; 28(1): 30-8, article.

Cheng AYY. Oral antihyperglycemic therapy for type 2 diabetes mellitus. Can Med Assoc J. 2005; 172(2): 213-26, CrossRef.

Rosita R, Yuandani Y, Marianne M. Nephroprotective activity of ethanol extract of Curcuma mangga val. in paracetamol-induced male mice. Asian J Pharm Clin Res. 2018; 11(13): 126, CrossRef.

Yuandani Y, Suwarso E. Immunomodulatory effects of ethanol extract of Curcuma mangga rhizomes in mice. Asian J Pharm Clin Res. 2017; 10(9): 148, CrossRef.

Kim JS, Lee SG, Kang YJ, Kwon TK, Nam JO. Kahweol inhibits adipogenesis of 3T3-L1 adipocytes through downregulation of PPARγ. Nat Prod Res. 2018; 32(10): 1216-9, CrossRef.

Paar M, Jüngst C, Steiner NA, Magnes C, Sinner F, Kolb D, et al. Remodeling of lipid droplets during lipolysis and growth in adipocytes. J Biol Chem . 2012; 287(14): 11164-73, CrossRef.

Rizzatti V, Boschi F, Pedrotti M, Zoico E, Sbarbati A, Zamboni M. Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution. Eur J Histochem. 2013; 57(3): 24, CrossRef.

Yen HF, Hsieh CT, Hsieh TJ, Chang FR, Wang CK. In vitro antidiabetic effect and chemical component analysis of 29 essential oils products. J Food Drug Anal. 2015; 23(1): 124-9, CrossRef.

Gaidhu MP, Ceddia RB. Remodeling glucose and lipid metabolism through AMPK activation: relevance for treating obesity and type 2 diabetes. Clin Lipidol. 2009; 4(4): 465-77, CrossRef.

Zhang D, Fu M, Gao SH, Liu JL. Curcumin and diabetes: a systematic review. Evidence-Based Complement Altern Med. 2013; 2013: 636053, CrossRef.

Ejaz A, Wu D, Kwan P, Meydani M. Curcumin inhibits adipogenesis in 3T3-L1 adipocytes and angiogenesis and obesity in C57/BL mice. J Nutr. 2009; 139(5): 919-25, CrossRef.

Feng S, Reuss L, Wang Y. Potential of natural products in the inhibition of adipogenesis through regulation of PPARγ expression and/or its transcriptional activity. Molecules. 2016; 21(10): 1278, CrossRef.

Yang H, Du Z, Wang W, Song M, Sanidad K, Sukamtoh E, et al. Structure–activity relationship of curcumin: role of the methoxy group in anti-inflammatory and anticolitis effects of curcumin. J Agric Food Chem. 2017; 65(22): 4509-15, CrossRef.

Sakuma S, Sumida M, Endoh Y, Kurita A, Yamaguchi A, Watanabe T, et al. Curcumin inhibits adipogenesis induced by benzyl butyl phthalate in 3T3-L1 cells. Toxicol Appl Pharmacol. 2017; 329: 158-64, CrossRef.




DOI: https://doi.org/10.21705/mcbs.v4i1.88

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