Comparison of Antiaging and Antioxidant Activities of Protocatechuic and Ferulic Acids

Ermi Girsang, I Nyoman Ehrich Lister, Chrismis Novalinda Ginting, Maulidwina Bethasari, Annisa Amalia, Wahyu Widowati


Background: Skin-aging is a progressive changes in the skin combine with cumulative extrinsic factors which are mostly caused by free radicals caused by exposure to lots of free radicals molecules from pollutant, wrongly food intake, or too much sun bathing. These free radicals can be tackled by a treatment using antioxidants. Prevention of aging can be done by escalating antioxidant intake. Protocatechuic acid (PCA) and Ferulic acid (FA) have been known for their scavenging properties on free radicals and antiaging activity. Antioxidant and antiaging activity of both compounds have not been compared comprehensively before. Hence, current study was conducted to compare the potential of PCA and FA for their antioxidant and antiaging activities using various methods.

Materials and Methods: Antioxidant analysis of PCA and FA was conducted using H2O2 scavenging assay, 2,2’-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazil (DPPH), and ferric reducing antioxidant power (FRAP). Meanwhile, antiaging activities of PCA and FA were examined using inhibitory activities of tyrosinase, collagenase, elastase, hyaluronidase and tyrosinase.

Results: IC50 of scavenging activity of ABTS were 125.18 µg/mL (PCA) and 35.55 µg/mL (FA), inhibition activity of collagenase were 126.16 µg/mL (PCA) and 52.85 µg/mL (FA) and inhibition activity of tyrosinase were 246.42 µg/mL (PCA), 253.58 µg/mL (FA).

Conclusion: In conclusion, FA has better ABTS scavenging and collagenase inhibition activities compared to PCA. Meanwhile, PCA has better activity of tyrosinase inhibition than FA.

Keywords: antioxidant, antiaging, ferulic acid, protocatechuic acid

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Hori M, Yagi M, Nomoto K, Shimode A, Ogura M, Yonei Y. Inhibition of advanced glycation end product formation by herbal teas and its relation to anti-skin aging. Anti-aging Med. 2012; 9(6): 135-48, article.

Baumann L. Skin ageing and its treatment. J Pathol. 2007; 211(2): 241–51, CrossRef.

Carola R, Harley JP, Noback CR. Human Anatomy and Physiology. 1st ed. New York: McGraw-Hill Publishing Company; 1990, article.

Mukherjee PK, Maity N, Nema NK, Sarkar BK. Bioactive compounds from natural resources against skin aging. Phytomedicine. 2011; 19(1): 64-73, CrossRef.

Kwak CS, Yang J, Shin C, Chung JH. Topical or oral treatment of peach flower extract attenuates UV-induced epidermal thickening, matrix metalloproteinase-13 expression and pro-inflammatory cytokine production in hairless mice skin. Nutr Res Pract. 2018; 12(1): 29-40. , CrossRef.

Kim J, Lee C, Kyung E, Lee S, Park N, Kim H, et al. Inhibition effect of gynura procumbens extract on uv-b-induced matrix-metalloproteinase expression in human dermal fibroblast. J Ethnopharmacol. 2011; 137(1): 427-33, CrossRef.

Chiang H, Chen H, Lin T, Shih I, Wen K. Michelia alba extract attenuates uvb-induced expression of matrix metalloproteinases via map kinase pathway in human dermal fibroblas. Food Chem Toxicol. 2012; 50(12): 4260-9,

Cho HPJ, Kim SHD, Cho HJIKY. Whitening and anti-wrinkle activities of ferulic acid isolated from tetragonia tetragonioides in B16F10 melanoma and CCD- 986sk fibroblast cells. J Nat Med. 2018; 72(1): 127-35, CrossRef.

Hong Y, Young E, Ouk D, Joo H. Physiological effects of formulation containing tannase-converted green tea extract on skin care: physical stability, collagenase, elastase, and tyrosinase activities. Integr Med Res. 2014; 3(1): 25-33, CrossRef.

Robbins RJ. Phenolic acids in foods: an overview of analytical methodology phenolic acids in foods. J Agric Food Chem. 2003; 51(10): 2866-87, CrossRef.

Liu RH. Nutrition and cancer potential synergy of phytochemicals in cancer prevention: mechanism of action 1. Int Res Conf Food. 2018; (February): 3479-85.

Hudson EA, Dinh PA, Kokubun T, Simmonds MSJ, Gescher A. Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells 1. Cancer Epidemiol Biomarkers Prev. 2000; 9(November): 1163-70, article.

Nile SH, Ko EY, Kim DH, Keum Y. Screening of ferulic acid related compounds as inhibitors of xanthine oxidase and cyclooxygenase-2 with anti-inflammatory activity. Rev Bras Farmacogn. 2016; 26(1): 50-5, CrossRef.

Kanski J, Aksenova M, Stoyanova A, Butterfield DA. Ferulic acid antioxidant protection against hydroxyl and peroxyl radical oxidation in synaptosomal and neuronal cell culture systems in vitro: structure – activity studies. J Nutr Biochem. 2002; 13(5): 273-81, CrossRef.

Saija A, Tomaino A, Proteggente A, Pas A De, Uccella N, Bonina F. Ferulic and caffeic acids as potential protective agents against photooxidative skin damage. J Sci Food Agric. 1999; 480: 476-80, CrossRef.

Kayano S, Kikuzaki H, Fukutsuka N, Mitani T, Nakatani N. Antioxidant activity of prune (Prunus domestica L.) constituents and a new synergist. J Agric Food Chem. 2002; 50(13): 3708-12. , CrossRef.

Li P, Q Wang X, Z Wang H, Wu Y. High Performance liquid chromatographic determination of phenolic acids in fruits and vegetables. Biomed Environ Sci. 1993; 6(4): 389-98, article.

Fitri A. Identification of Phytochemical and Antioxidant Activity in Peel and Seed of Tropical Fruits from Indonesia. Bogor: Institut Pertanian Bogor; 2015, article.

Kakkar S, Bais S. A Review on protocatechuic acid and its pharmacological potential. Pharmacology. 2014; 2014: 952943, CrossRef.

Utami S, Adityaningsari P, Sosiawan I, Endrini S, Romadhiyani Q. Antioxidants and anticholinesterase activities of the characterized ethanolic of ripe sesoot (Garcinia picrorrhiza Miq.) fruit extract (GpKar) and xanthone. Tradit Med J. 2017; 22(December): 160-5, CrossRef.

Widowati W, Janeva WB, Nadya S, Amalia A, Arumwardana S, Kusuma HSW, et al. Antioxidant and antiaging activities of Jasminum sambac extract, and its compounds. J Rep Pharmaceut Sci. 2018; 7(3): 270-85, article.

Widowati W, Fauziah N, Herdiman H, Afni M, Afifah E, Sari H. Antiepileptic and effects antioxidant and anti aging assays of oryza in acid sativa extracts, vanillin and coumaric acid. J Nat Remedies. 2016; 16(3): 1-12, CrossRef.

Widowati W, Rani AP, Hamzah RA, Arumwardana S, Afifah E. Antioxidant and antiaging assays of Hibiscus sabdariffa extract and its compounds. Nat Prod Sci. 2017; 23(3): 192-200, CrossRef.

Widowati W, Wargasetia TL, Afifah E, Mozef T, Kusuma HSW, Nufus H, et al. Antioxidant and antidiabetic potential of Curcuma longa and its compounds. Asian J Agric Biol. 2018; 6(2): 149-61, article.

Thi P, Tu B, Tawata S. Anti-oxidant, anti-aging, and anti-melanogenic properties of the essential oils from two varieties of Alpinia zerumbet. Molecules. 2015; 20: 16723-40. , CrossRef.

Inês M, João M, Alves RC, Ferreira ICFR. Exploring plant tissue culture to improve the production of phenolic compounds: a review. Ind Crop Prod. 2015; 2015: 1-14, CrossRef.

Sofic E, Lange KW, Jellinger K, Riederer P. Reduced and oxidized glutathione in the substantia nigra of patients with parkinson’s disease. Neuroscience. 1992; 142(2): 128-30, CrossRef.

Tian L, Cai Q, Wei H. Alterations of antioxidant enzymes and oxidative damage to macromolecules in different organs of rats during. Free Radic Biol Med. 1998; 24(9): 1477-84, CrossRef.

Buettner GR. The pecking order of free radicals and antioxidants: lipid peroxidation, a-tocopherol, and ascorbate. Arch Biochem Biophys. 1993; 300(2): 535-43, CrossRef.

Newmark HL. Plant phenolic compounds as inhibitors of mutagenesis and carcinogenesis. Am Chem Soc. 1992; 507(4): 48-52, CrossRef.

Huang M, Ferraro T. Phenolic compounds in food and cancer prevention. Am Chem Soc. 2000; 507(2): 8-34, CrossRef.

Cini M, Fariello RG, Bianchetti A, Moretti AI. Studies on lipid peroxidation in the rat brain. Neurochem Res. 1994; 19(3): 283-4, CrossRef.

Tseng T, Kao T, Chu C, Chou F, Lin W, Wang C. Induction of apoptosis by hibiscus protocatechuic acid in human leukemia cells via reduction of retinoblastoma (rb) phosphorylation and bcl-2 expression. Biochem Pharmacol. 2000; 60(00): 307-15, CrossRef.

Ho Y, Magnenat J, Gargano M. The nature of antioxidant defense mechanisms: a lesson from transgenic studies. Environ Health Perspect. 1998; 106 (Suppl 5): 1219-28, CrossRef.

Zhang Z, Chang Q, Zhu M, Huang Y, Ho WKK, Chen Z. Characterization of antioxidants present in hawthorn fruits. J Nutr Biochem. 2001; 12(3): 144-52, CrossRef.

Zhang X, Shi GF, Liu X, An L, Guan S. Anti-ageing effects of protocatechuic acid from alpinia on spleen and liver antioxidative system of senescent mice. Cell Biochem Funct. 2011; 29(April): 342-7, CrossRef.

Truong XT, Park S, Lee YG, Jeong HY, Moon JH, Jeon TI. Protocatechuic acid from pear inhibits melanogenesis in melanoma cells. Int J Mol Sci. 2017; 18(8): pii: E1809, CrossRef.

Pluemsamran T, Onkoksoong T, Panich U. Caffeic acid and ferulic acid inhibit UVA-induced matrix metalloproteinase-1 through regulation of antioxidant defense system in keratinocyte HaCaT cells. Photochem Photobiol. 2012; 88(4): 961-8, CrossRef.

Kumar N, Pruthi V. Potential applications of ferulic acid from natural sources. Biotechnol Reports. 2014; 4: 86-93, CrossRef.

Aqil F, Munagala R, Jeyabalan J, Joshi T, Singh IP. The Indian blackberry (jamun), antioxidant capacity, and cancer protection. cancer: oxidative stress and dietary antioxidants. In: Cancer: Oxidative Stress and Dietary Antioxidants. Atlanta: Elsevier; 2014. p.101-13, CrossRef.

Li X, Wang X, Chen D, Chen S. Antioxidant activity and mechanism of protocatechuic acid in vitro xican. Funct Foods Heal Dis. 2011; 1(7): 232-44, CrossRef.


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Cell and BioPharmaceutical Institute