Density of Dermatophagoides spp. and Its Relationship with House-dust Mite Specific Serum IgE in Persistent Asthma

Annisa Mulia Anasis, Anna Rozaliyani, Heri Wibowo

Abstract


Background: Asthma is a chronic inflammation of the bronchial tree that emerges as a response to exogenous factors, such as allergens, irritants, and infections. Some asthmatic patients had been reported having symptoms of asthma due to house-dust mites (HDM) allergen exposure. It is associated with immune responses which were increased in the form of specific Immunoglobulin E (IgE) production against HDM allergens. This case-control study aimed to determine the HDM profiles in persistent asthmatic patients, including density of mites, as well as its relationship with specific IgE anti-HDM serum levels.

Materials and Methods: A total of 13 patients with persistent asthma and 12 control patients had their specific anti-HDM IgE levels examined using Immulite 2000 xpi. The house dust samples were taken and analyzed with the Fain method.

Results: The results have shown that 69% of patients in the persistent asthma group and 25% of normal patients were positive for IgE anti-HDM. Dermatophagoides pteronyssinus is a predominant species with a total of 120 mites (83.9%) of 143 mites. Correlation analysis indicated a positive relationship between IgE anti-HDM levels within the serums of patients and the density of mites in the dust obtained from bedroom spaces (Spearmen Rho, R=0.35, p=0.04).

Conclusion: Positive IgE anti-HDM patients in the persistent asthma group were higher (69%) than those in the clinically normal group (25%). The density of mites were dominated by D. Pteronyssinus. The bedroom-dust mites density revealed a positive correlation with serum IgE anti-HDM levels in persistent asthma patients.

Keywords: asthma, density, Dermatophagoides spp.,IgE


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References


Lynden-van Nes AMT van, Koren LGH, Snijders MCL, Bronswijk JEMH van. Medical impact of arthropod allergens. In: Wildey KB, editor. The 2nd International Conference on Insect Pests in the Urban Environment. Exeter: BPC Wheatons; 1996. p.17-30.

Baxi SN, Phipatanakul W. The role of alergen exposure and avoidance in asthma. Adolesc Med State Art Rev. 2010; 21(1): 57-71, article.

Sari CYI. Inflamasi alergi pada asma. CDK. 2013; 40(8): 585-8.

Faiza A. Hubungan antara lama penggunaan kasur kapuk dengan jumlah tungau debu rumah di perumahan PJKA Kelurahan Randusari Semarang. Semarang: Universitas Diponegoro; 2006, article.

Purba ISE, Pijoh VD, Runtuwene J. Survey perilaku masyarakat terhadap populasi tungau debu rumah di Kelurahan Titiwungen Selatan Kecamatan Sario Kota Manado. Jurnal e-Biomedik. 2013; 1(1): 319-24, CrossRef.

Natalia D. Peranan alergen tungau debu rumah (Der p 1 dan Der p 2) dalam reaksi alergi. CDK. 2015; 42(4): 251-5, article.

Fernández-Caldas E, Inmunotek SL, Puerta L, Caraballo L, Lockey RF. Mite allergens. In: Allergens and Allergen Immunotherapy. Florida: CRC Press; 2014. p.181-201, CrossRef.

Yu SJ, Liao EC, Tsai JJ. House dust mite allergy: environment evaluation and disease prevention. Asia Pac Allergy. 2014; 4(4): 241-52, CrossRef.

Bakolis I, Heinrich J, Zock JP, Norback D, Svanes C, Chen CM, et al. House dust-mite alergen exposure is associated with serum specific IgE but not with respiratory outcomes. Indoor Air. 2015; 25(3): 235-44, CrossRef.

Arlian LG, Thomas AE. The biology of dust mites and the remediation of mite allergens in allergic disease. J Allergy Clin Immunol. 2001; 107(3): S406-13, CrossRef.

Calderon MA, Linneberg A, Tebbe JK, Blay FD, Rojas DH, Virchow JC. Respiratory allergy caused by house dust mites: what do we really know?.J Allergy Clin Immunol. 2015; 136(1): 38-48, CrossRef.

Vallance G, McSharry C, Wood S, Thomson NC. House dust mite control measures in the treatment of asthma. Ther Clin Risk Manag. 2006; 2(4): 347-54, CrossRef.

Customer Education and Communications. IMMULITE® 2000/2500 operators. Erlangen: Siemens Medical Solutions Diagnostics; 2007, article.

Aulung A, Djaja B, Widjaja M, Tjokronegoro A. Penggunaan teknik isolasi pada pemeriksaan tungau debu rumah. Medika. 1986: 2: 161-5.

Susanto AJ, Rengganis I, Rumende MC, Harimurti K. The differences in serum quantitative specific IgE levels induced by Dermatophagoides pteronyssinus, Dermatophagoides farinae and Blomia tropicalis sensitization intermittent and persistent allergic asthma. Acta Med Indones. 2017; 49(4): 299-306, article.

Yu JM, Luo QH, Sun JL, Shi CL, Yin J, Zhou YL, et al. Diversity of house dust mite in Xishuangbanna Dai, a tropical rainforestregion in Southwest. BioMed Research International. 2015: 2015; 421716, CrossRef.

Zhang C, Li J, Lai X, Zheng Y, Gjesing B, Spangfort MD, Zhong N. House dust mite and tungau gudang IgE reactivity in allergic patients from Guangzhou, China. Asian Pac J Allergy Immunol. 2012; 30(4): 294-300, article.

Zeytun E, Dogan S, Ozcicek F, Unver E. Sensitivity to house dust mites alergens in patients with allergic asthma in Erzincan Province, Turkey. Turkiye Parazitol Derg. 2017; 41(1): 34-41, CrossRef.

Sundaru H. House dust mite alergen level and alergen sensitization as risk factors for asthma among student in Central Jakarta. Med J Indo. 2006; 15(1): 55-9, CrossRef.




DOI: https://doi.org/10.21705/mcbs.v4i2.92

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